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Structural Biology 2018
Volume: 4
Biochemistry & Molecular Biology Journal
Page 57
March 15-16 2018
Barcelona, Spain
10
th
Edition of International Conference on
Structural Biology
C
hiral antifungals are used as veterinary and human drugs, as
well as agrochemicals. They are especially used to cure fungal
infections that commonly occur on the nails, hair and skin. Those
antifungals inusewhichshowefficacyagainst fungal diseasesare
mostly target specific components of fungal plasma membrane
or its biosynthetic pathways. Enantioselectivity of chiral imidazole
and triazole derivatives in biological action, metabolism and
pharmacokinetics is well documented. In this work, separation
of enantiomers of some chiral antifungals was studied on two
polysaccharide-based chiral columns by high performance liquid
chromatography (HPLC) using polar organic mobile phases
such as methanol, acetonitrile, ethanol and isopropanol. 10
chiral antifungal pharmaceuticals (ketoconazole, sertaconazole,
miconazole, terconazole, tioconazole, isoconazole, fluconazole,
luliconazole, posaconazole and itraconazole) were separated
by Lux i-Cellulose 5 (cellulose 3,5-dichlorophenylcarbamate)
and one experimental amylose based chiral column (amylose
3,5-dimethylphenylcarbamate) with the dimension 4.6x250 mm.
The mobile phase flow rate was 1 mL/min and the temperature
of the columns was kept at 25
°
C. Polar organic mobile phases
offer certain advantages for separation of enantiomers such
as short analysis time, high plate numbers and favorable
signal to noise ratio. Used chiral columns offer good stability in
pure polar organic solvents as well as wider variety of mobile
phases. Finally, this study demonstrated the chiral columns
based on cellulose 3,5-dichlorophenylcarbamate and amylose 3,
5-dimethylphenylcarbamate covalently attached to the surface of
silica particles can effectively separate the chiral imidazole and
triazole derivatives by using polar organic mobile phases.
bounoua_nadia@yahoo.frHPLC separation of enantiomers of some antifungals using
covalently immobilized polysaccharide‑based chiral selectors
and polar organic mobile phases
Nadia Bounoua
1,3
, Khaled Kekkoum
1
, Mehmet Gumustas
2,3
Nasser Belboukhari
1
,
Sibel A Ozkan
2
and
Bezhan Chankvetadze
4
1
University of Béchar, Algeria
2
Hitit University, Turkey
3
Ankara University, Turkey
4
Tbilisi State University, Georgia
Biochem Mol biol J, Volume 4
DOI: 10.21767/2471-8084-C1-009