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Structural Biology 2018
Volume: 4
Biochemistry & Molecular Biology Journal
Page 54
March 15-16 2018
Barcelona, Spain
10th Edition of International Conference on
Structural Biology
Bovineviral diarrheavirus (BVDV) isgeneticallyandserologically
related to other members of the genus Pestivirus, such as
classical swine fever virus, andmay cause reproductive problems,
but there is still a lack of research to determine the pathogenicity
in different gestational periods of sows (pigs) and consequences
in newborn piglets. The objective of this work was the analysis
of the effect of bovine viral diarrhea virus infection in gestation
and in swine neonates. Twelve pregnant sows divided into five
groups were used, which were inoculated experimentally with
the BVDV-2 strain, one group with 30 days before insemination
(G0); three groups during gestation, first (G1), second (G2) and
last third (G3); and the fifth control group (G4). Samples of blood,
serum and nasal swabs were collected every three days from the
day of inoculation through the farrowing. On the day of delivery,
half of the newborns were euthanized to obtain blood and organ
samples at necropsy. The collected samples were analyzed by
means of the virus neutralization, real time RT-PCR, blood count
and histopathology. The RT-qPCR was performed using the
TaqMan system, Thermofisher™-VetMAX™-Gold BVDV Detection
Kit commercial kit. Serial dilutions of VS253 were diluted at 100
and 107 to detect the limit of RT-qPCR. All sows seroconverted
during the gestational period, except the control group and BVDV-
2 was detected in blood and nasal swabs in G0, G1, G2 and G3
sows with a Ct<36, the viremia was detected from day 3 post-
infection (dpi) through 12 dpi and viral shedding was detected
from 6 dpi through 23 dpi, and the piglets developed gliosis. G1
sows presented thrombocytopenia at day 36 after inoculation.
The dynamics of BVDV-2 infection in pigs was clarified, such as
the serological and viremic profile, shedding phase and clinical
characteristics; however, transplacental virus transmission was
not detected.
Biography
Pereira D A is pursuing her PhD at the University of São Paulo, Brazil. Her
expertise is in the health of swine. She works on laboratory techniques
such as molecular and immunodiagnostics for swine diseases, especially
RNA and virus (Pestivirus). She graduated in Veterinary Medicine in 2009.
She received a scholarship from FAPESP (Brazilian Founding: process:
2015/08531-0) and has over 9 publications. She has also been serving as a
member of Suinesp (Group of studies in swines).
Experimental inoculation in pregnant sows with bovine viral
diarrhea virus 2
Pereira D A, Oliveira L G, Souza H M A, Baraldi T G and Kasmanas T C
University State of São Paulo, Brazil
Pereira D A et al., Biochem Mol biol J, Volume 4
DOI: 10.21767/2471-8084-C1-009