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Structural Biology 2018
Volume: 4
Biochemistry & Molecular Biology Journal
Page 58
March 15-16 2018
Barcelona, Spain
10
th
Edition of International Conference on
Structural Biology
A
mong the diseases of the blood, thalassemia occupies
a special place, associated with a reduction or complete
absence of synthesis of globin chains of hemoglobin. Azerbaijan
is considered as an endemic zone of these inherited blood
diseases, which makes conducted researches relevant. The aim
of this work was to study the relationship between the thiol status
of blood and the secretion of endogenous antimicrobial peptides.
The blood of 57 patients aged 6-17 years was studied. All patients
dependingon thepathologyweredivided intothe followinggroups:
group I-20 children with a homozygous form of
β
-thalassemia,
group II-37 children with G6PD deficiency. To assess the degree
of oxidative stress of the body, carbonylated proteins (CP) and
thiol status (TS) of blood were chosen as markers. To assess
the level of secretion of endogenous antimicrobial peptides, a
quantitative analysis of defensin and endotoxin in blood plasma
was performed using the ELISA method. The research was
carried out with the financial support of the Science Development
Foundation of Azerbaijan. As a result of research, it was revealed
that in group I patients, the amount of CP increased by 11%, in the
group II patients CP increased by 1.6% and TS decreased by 1.5%.
The level of defensin in group I increased by 2%, and endotoxin
by 1.7%. In group II, these indicators increased by 1.7% and 2.3%,
respectively. With the change of the body’s TS, the secretion
of
α
-defensin was increasing. In
β
-thalassemia, carbonylated
proteins increase in the blood, thiol status decreases, which
indicates at the increase of the influence of oxidative stress
associated with frequent infectious complications and activation
of neutrophils.
gulib18@mail.ruInfluence of the oxidative stress on the secretion of the
endogenous antimicrobial peptides in hereditary blood diseases
Azizova G I, Dadasheva A R
and
Efendiyev A M
Azerbaijan Medical University, Azerbaijan
Biochem Mol biol J, Volume 4
DOI: 10.21767/2471-8084-C1-009