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Structural Biology 2018
Volume: 4
Biochemistry & Molecular Biology Journal
Page 61
March 15-16 2018
Barcelona, Spain
10
th
Edition of International Conference on
Structural Biology
T
he aimof the study is to analyze
in silico
,
the conjoint action of
acetylcholine (ACh), nitric oxide (NO) and high concentrations
of motilin (Mot) on myoelectrical activity of the gastric antrum
under complex physiological stimuli. Acting alone at increasing
doses, Mot steadily depolarizes smooth muscle, reduces the
amplitude and shortens the duration of slow waves. These
changes correspond to a significant rise in the basal muscle tone
and the active force, T
a
=16.1 mN/cm. The mechanical stretching
of the antrum at a high frequency and the subsequent release of
Ach, results in the production of regular high amplitude spikes
on the crests of slow waves. Smooth muscle responds with
strong phasic contractions, T
a
=8.3 mN/cm. The application of
Mot at 50-100 nM does not affect the cholinergically mediated
myoelectrical activity, although it evokes contractions of
inconsistent amplitudes: T
a
=2.9-8.3 (mN/cm). The release of a
“puff” of NO to the gastric antrumwhich has been exposed to ACh
and Mot, fails to exert any inhibitory effect. When the addition of
NO precedes ACh and Mot, acute short-lasting relaxations with
min T
a
=7.7 mN/cm are observed. The asynchrony between the
firing rate of interstitial cells of Cajal and the presence of Mot
at 85 nM causes the production of active forces of wavering
strength. The antrum fails to relax completely. A lower frequency
of ganglionic activity allows a greater degree of relaxation,
T
a
=8
mN/cm, and contractions of larger amplitude, 9.9 mN/cm. The
results have unveiled intricacies of co-transmission by multiple
neurotransmitters in the antrum of the human stomach and
the dynamics of active forces development. The chronotropic
allosteric interaction among ACh, NO, Mot and interstitial cells of
Cajal plays a pivotal role in coordinatedmotor activity of the organ.
Abnormalities in their interplay could lead to motor dysfunction.
saleh2030bh@gmail.comComputer simulation of multiple neurotransmission in the
human gastric antrum
Saleh Rowaili
and
Roustem Miftahof
Arabian Gulf University, Bahrain
Biochem Mol biol J, Volume 4
DOI: 10.21767/2471-8084-C1-009