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Structural Biology 2018
Volume: 4
Biochemistry & Molecular Biology Journal
Page 65
March 15-16 2018
Barcelona, Spain
10
th
Edition of International Conference on
Structural Biology
R
ecently, we showed that autophagy receptor p62/SQSTM-1
assembles into flexible helical filaments. In the current talk,
we provide further detailed insights into the molecular basis of
polymer formation. Using EM based structure elucidation
in vitro
and
in situ
reveals large oligomeric and polymeric cargo receptor
complexes giving rise to higher-order structures that constitute
the scaffold for autophagosome formation. The organization
of small receptor proteins into helical assemblies provides a
cellular mechanism for high selectivity in cargo recognition and
a fundamental architecture that enables cargo encapsulation
of various sizes from molecular to cellular scale. The presented
example illustrates the versatility and synergy of structural and
cellular electron microscopy approaches.
carsten.sachse@embl.deInsights from oligomeric and polymeric autophagy receptor
complexes by electron microscopy
Sachse C
EMBL Heidelberg, Germany
Biochem Mol biol J, Volume 4
DOI: 10.21767/2471-8084-C1-009