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Page 68
Volume 4, Issue 2 American Journal of Ethnomedicine
ISSN 2348-9502
Natural Products Congress & World Pharma Congress 2017
October 16-18, 2017
3rd World Congress on
NATURAL PRODUCTS CHEMISTRY AND RESEARCH
&
12th WORLD PHARMA CONGRESS
October 16-18, 2017 Budapest, Hungary
Therapeutic effects of crude and degummed Citrullus lanatus seed oil on candidiasis in
immunosuppressed rats
Victor Onukwube Apeh
University of Nigeria, Nigeria
Vegetable oil has become an integral part of our diet, but relatively little is known about their antifungal potential. The
present work was designed to evaluate the therapeutic efficacy of crude and degummed Citrullus lanatus (Watermelon)
seed oil (CLSO) in the treatment of experimental oral candidiasis induced by Candida albicans in immunosuppressed rats. This
anticandidal activity was analyzed by microbiological and histopathological techniques at days 7 and 14; it was compared with
that of fluconazole, which was used as a positive control. Microbiologically, CLSO significantly (p<0.05) reduced the number of
Colony Forming Units (CFU) sampled from the kidney tissue of rats treated for fourteen consecutive days, compared to group
3 (untreated control) rats. Treatment with fluconazole gave similar results at day 7 but exacerbated at day 14. Histologically,
group 3 showed multifocal aggregation and widespread distribution of fungal blastospores (arrow) appearing singly or in small
clusters within the renal parenchyma. While minimal fungal blastospores present in the renal parenchyma; fungal blastospores
appearing singly within the glomeruli and renal tubules was seen in CLSO-treated animals, but minimal blastospore was less
in degummed CLSO than in crude CLSO. Thus, the histological data were confirmed by the microbiological tests for crude
and degummed CLSO, but not for the fluconazole-treated group which showed multifocal and widespread distribution of
blastospores at day 14. Therefore, CLSO could be considered as an antifungal agent with degummed CLSO being more potent
and could be proposed as therapeutic agents for oral candidiasis.
Urtica dioica distillate (Aragh Gazaneh ) restores altered glucose metabolism in diabetic rats
A Seghatoleslam 1,2 , M Akmali 2 , F Zamani-Garmsiri 2, A Gohari 2
1,2 Shiraz University of Medical Sciences, Shiraz, Iran
Background: Urtica dioica (UD) is well known as a hypoglycemic plant. While the anti-diabetic properties of its extract is
well studied, there are not any published reports regarding its distillate, a drink widely being used in different areas of Iran
according to Traditional Iranian Medicine for treating diabetic patients.
Materials and methods: To justify the use of UD distillate (UDD) for treatment of diabetes, a series of experiments were
performed on 24 male rats. The groups consisted of two treatment and two control groups, each one containing normal and
diabetic rats. During 4 weeks, the rats in the treatment and control groups received UDD and water by gavage, respectively.
Every nine days, the rats were weighted and their fasting blood glucose (FBS) values were measured. Following 4 weeks of
treatment, all the rats were sacrificed for further experiments. FBS, serum insulin levels and the specific activity of hepatic
enzymes including glucokinase, hexokinase and glucose 6-phosphate dehydrogenase were measured using standard methods.
Results and discussion: The amount of insulin secretion and also the specific activities of hepatic enzymes were significantly
increased in the treated diabetic group. A significant decrease was also observed in the blood glucose of the treated diabetic
rats compared to the diabetic control ones. UDD consumption by diabetic treated rats not only prevented weight loss but also
caused a dramatic weight gain. Therefore, these results suggested that UDD administration could improve diabetic conditions
by enhancing insulin secretion and liver glucose metabolizing enzymes’ activity and could be used as an anti-diabetic drink
as well.
American Journal of Ethnomedicine, 4:2
DOI: 10.21767/2348-9502-C1-003