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Volume 4, Issue 2 American Journal of Ethnomedicine
ISSN 2348-9502
Natural Products Congress & World Pharma Congress 2017
October 16-18, 2017
3rd World Congress on
NATURAL PRODUCTS CHEMISTRY AND RESEARCH
&
12th WORLD PHARMA CONGRESS
October 16-18, 2017 Budapest, Hungary
Isolation of male sterile and maintainer lines from North Indian onion (Allium cepa L.) populations
with the aid of PCR based molecular marker
Geetika Malik1, Ajmer Singh Dhatt2 and Ajaz Ahmed Malik3
1ICAR-Central Institute of Temperate Horticulture, India
2Punjab Agricultural University, India
3Sher-e-Kashmir University of Agricultural Sciences and Technology, India
Marker Assisted Selection (MAS) using mitochondrial DNA based marker cytochrome b ( cob) was integrated with phenotypic
evaluation to isolate male sterile and maintainer lines from open pollinated onion varieties adapted to North Indian agro-
climatic region. Cytotype (N/S) determination by cob marker followed by morphological and microscopic study of pollen discovered
male sterile plants (Smsms) at frequencies of 0.015 in Punjab Naroya, 0.020 in Punjab Selection, and 0.006 in Punjab White. The
progeny scoring of test-crosses between male sterile and N-cytoplasmic plants isolated the maintainers (Nmsms) at frequencies of
0.133 in Punjab Naroya, 0.231 in Punjab Selection and 0.182 in Punjab White. As a novel approach, Trait Recovery Programme was
demonstrated to reduce the population size required to recover a male sterile plant by 91.08% in Punjab Naroya, 92.99% in Punjab
Selection and 97.66% in Punjab White. For recovering a maintainer, 10% reduction in Punjab Naroya and 9.10% in Punjab Selection
was calculated. However, no reduction was observed in Punjab White. This analysis also validated that in a randomly mating onion
population, frequency of recessive ms allele squared is equal to the frequency of male sterile plants among S-cytotype and frequency
of maintainers among N-cytotype (fms2 = fSmsms/fS = fNmsms/fN).
Biography
Geetika Malik is currently working as an Scientist and Assistant Professor in the division of vegetable science at Sher-e-Kashmir University of Agricultural Sciences
and Technology of Kashmir, India. She is expertise in Natural Products and Horticulture.
Geetika Malik et al., American Journal of Ethnomedicine, 4:2
DOI: 10.21767/2348-9502-C1-003