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Volume 4, Issue 2
American Journal of Ethnomedicine
ISSN 2348-9502
Natural Products Congress & World Pharma Congress 2017
October 16-18, 2017
3
rd
World Congress on
NATURAL PRODUCTS CHEMISTRY AND RESEARCH
&
12
th
WORLD PHARMA CONGRESS
October 16-18, 2017 Budapest, Hungary
Analysis of genetic diversity of Indian melon (
Cucumis melo L
.) land races and its comparison with
global reference melon populations
Ajaz A Malik
1
, N P S Dhillon
2
and
Geetika Malik
3
1
Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu and Kashmir, India
2
AVRDC - The World Vegetable Center, Thailand
3
Central Institute of Temperate Horticulture, India
T
he present investigation entitled, “Analysis of genetic diversity of Indian melon (
Cucumis melo L.)
land races and its comparison
with global reference melon populations” was conducted at Department of Vegetable Science and School of Agricultural
Biotechnology, Punjab Agricultural University Ludhiana. Eighty-eight melon accessions collected from Uttrakhand and Uttar
Pradesh states of India representing four agro-ecological regions (six sub-regions) and eight reference accessions from USA were
characterized and evaluated for nineteen morphological traits of plant and fruit, biochemical traits such as T S S, ascorbic acid
content, titrable acidity and dry matter content, SSR genotyping and reaction to diseases. Significant differences were noted among
all the accessions for all the characters observed. Phenotypic and genotypic coefficients of variation were found to be high for fruit
weight and node at which first hermaphrodite flower appears. High heritability alongwith high genetic advance was recorded for
fruit weight, node at which first hermaphrodite flower appears, fruit length, seed cavity length, number of primary branches per vine
and total soluble solids content. D2 analysis grouped the accessions into ten clusters. The reference accessions obtained from USA
and land races collected from different agro-ecological zones of India were found to be scattered in different clusters. No parallelism
was found between genetic and geographic diversity. DNA polymorphism was utilized to cluster the genotypes into different clusters
based on similarity as well as dissimilarity coefficients. On basis of SSR analysis, dendrogram clustered 96 accessions into three
major groups. There was a significant correlation between botanical groups and the clustering pattern. Accessions belonging to
cantalupensis cluster together in cluster I, accessions of reticulatus group cluster together in cluster II and momordica group cluster
together in cluster III. However, some accessions of cantalupensis and reticulatus were intermixed in cluster I and II. Reference
accessions cluster together forming a genetically unique assemblage in sub-group IIA and shared similarity coefficient of 0.65
with sub-group IIB. This suggested that reference accessions shared genetic affinities with Indian melon accessions that could not
have been predicted based on their geographic origin. Four accessions were free from CMV and two accessions exhibited immune
reaction to downy mildew. The results inferred that these melon accessions could be used to broaden the genetic base of melon.
Ajaz A Malik et al., American Journal of Ethnomedicine, 4:2
DOI: 10.21767/2348-9502-C1-003
Notes: