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Page 77
Volume 4, Issue 2
American Journal of Ethnomedicine
ISSN 2348-9502
Natural Products Congress & World Pharma Congress 2017
October 16-18, 2017
3
rd
World Congress on
NATURAL PRODUCTS CHEMISTRY AND RESEARCH
&
12
th
WORLD PHARMA CONGRESS
October 16-18, 2017 Budapest, Hungary
Standardization study of oral drops formula from
Paronychia argentea
extract
Suleiman Olimat
Jordan University of Science and Technology, Jordan
P
aronychia argentea
has been proved to show hypoglycemic activity and is useful as gastric analgesic, in bladder, prostate,
abdominal ailments treatment, and stomach ulcers treatment has. Vanillic acid and luteolin are considered as the major
active constituents of
P. argentea
responsible for its biological activity. The aim is to develop an oral drop formula from the
plant extract depending on a HPLC method for standardization of
P. argenteae
, depending on vanillic acid as a main efficacious
active constituent in the plant. 76 g dried plant was extracted with 1 liter methanol. A tiny peak of vanilic acid was shown at
7.6 min, although statistically significant propylene glycol proved to be better extraction solvent for vanilic acid producing
major peak with higher response (AUC). Linearity of the assay method was evaluated by determining concentration levels
from concentration (0.4–4 µg mL
-1
); correlation coefficient obtained was 0.995. The best-fit linear equation obtained was
Y=7e
7
X-9817. The precision of the vanillic acid peak in the test solution was checked by injecting six individual preparations
prepared according to the description mentioned in method of analysis.
smalkhalil@just.edu.joAmerican Journal of Ethnomedicine, 4:2
DOI: 10.21767/2348-9502-C1-003