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Volume 4, Issue 2
American Journal of Ethnomedicine
ISSN 2348-9502
Natural Products Congress & World Pharma Congress 2017
October 16-18, 2017
3
rd
World Congress on
NATURAL PRODUCTS CHEMISTRY AND RESEARCH
&
12
th
WORLD PHARMA CONGRESS
October 16-18, 2017 Budapest, Hungary
Antihypertensive activity and standardization of the bioactive fraction of hyphaenethebaica growing
in Egypt
Noha A Khalil
1
, Amira Abdel Motaal
2
, K M Meselhy
2
and
Soad M Abdel khalek
3
1
Misr International University, Egypt
2
Cairo University, Egypt
3
Helwan University, Egypt
Background
: Hyphaene thebaica herb is well known in Egypt for its antihypertensive activity. However a standardized herbal
extract of Hyphaene thebaica has never been prepared in a pharmaceutical dosage form.
Methods
: A biologically guided fractionation was carried out
in-vitro
for the 50% and 70% ethanol extracts of Hyphaene
thebaica herb using the Angiotensin Coverting Enzyme (ACE) inhibition assay and renin inhibition assay. A validated reversed
phase HPLC method was developed for the standardization of the active fractions.
Results
: The ethyl acetate fraction of the 70% ethanol extract contained higher percentages of the three compounds chlorogenic
acid, quercetin and apigenin (1.940%, 2.994% and 0.612%, respectively) relative to the ethyl acetate fraction of the 50% ethanol
extract (1.384%, 0.342% and 0.070%, respectively). Also by comparing all fractions, the butanol fraction of the 70% ethanol
extract showed the highest ACE inhibition activity (IC50= 0.001436) and the highest renin inhibition activity (%inhibition=
93.69% at concentration 0.5 mg/ml). A standard calibration curve for the three compounds was established at a concentration
range of 0.1-50 μg/ml and it showed good linearity with a correlation coefficient (R2) of (1, 1 and 0.999, respectively). A high
degree of precision (relative standard deviation values <5%) was achieved. The limits of detection for the three compounds
were 0.428, 0.368 and 0.849 respectively. While the limits of quantification for the three compounds were 1.29, 1.11 and 2.57
respectively.
Conclusion
: Current results showed that the butanol fraction of the 70% ethanol extract revealed the highest antihypertensive
activity through anACE inhibitionmechanismand renin inhibitionmechanism. In addition, recorded observations concerning
linearity of the used bioactive markers offer a support for the possible utility of the tested extracts as potent standardized
antihypertensive drugs.
noha.alaa@miuegypt.edu.egAmerican Journal of Ethnomedicine, 4:2
DOI: 10.21767/2348-9502-C1-003