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Volume 4, Issue 2
American Journal of Ethnomedicine
ISSN 2348-9502
Natural Products Congress & World Pharma Congress 2017
October 16-18, 2017
3
rd
World Congress on
NATURAL PRODUCTS CHEMISTRY AND RESEARCH
&
12
th
WORLD PHARMA CONGRESS
October 16-18, 2017 Budapest, Hungary
Chemical study, antioxidant analysis and evaluation of the larvicidal potential against
Aedes Aegypti
Larvae
of essential oil of
Ocimum Basilicum Linn
Sheylla SMS Almeida
UNIFAP, Brasil
T
he purpose of this research was to accomplish chemical study, antioxidant analysis and evaluation of the larvicidal
potential against
Aedes aegypti larvae
of essential oil from the leaves of
O. basilicum Linn
. The research was carried out
in the Pharmacognosy and Phytochemistry Laboratory, Department of Biologicaland Health Sciences, Federal University
of Amapá (UNIFAP), between July 2013 and March 2014. Arthropoda Laboratory, Department of Biological and Health
Sciences, Federal University of Amapá (UNIFAP) between September 2013 and March 2014. The essential oil was obtained
by hydrodistillation; the identification and quantification of components was achieved with the use of GC-MS analysis. The
antioxidante activity was evaluated by the method of sequestration of DPPH. The essential oil was tested in the third larval
state of the development of the mosquito Aedes Aegypti. The third larval instar were exposed to different concentrations of the
oil (500, 400, 300, 200 and 130 ppm) in triplicates. Chromatographic analysis identified that the major constituents found in
essential oil of
O. basilicum
were limonene (13%), 1,8-cineole (15%), linalool (20%) and methyl chavicol (45%). In trials of free
radicals sequestration, the essential oil showed (AA%) 67.35±1.11 in the highest concentration and inhibitory concentration,
IC50 value of 61.517 mg/mL. The essential oil of
O. basilicum
showed larvicidal potential with CL50 of 67.22 ppm. A more
detailed study should be done to verify the larvicidal potential and biological mechanism of action, as several authors claimed
that the constituent of essential oils affect the nervous system of the mosquito Aedes Aegypti and the action mechanism is not
yet fully elucidated. New studies demand the development of tests using samples of lower concentrations to verify the degree
of toxicity in other animal species, including man, and preparation of formulations that may function as a natural alternative
to combat mosquito larvae.
sheyllasusan@yahoo.com.brPiroxicam loaded solid lipid nanoparticles for topical delivery: Preparation, characterization and
in
vitro
permeation assessment
Soliman Mohammadi-Samani
1
, Shirin Zojaji
2
and
Elaheh Entezar-Almahdi
1
1
Shiraz University of Medical Sciences, Iran
2
International Branch of Shiraz University of Medical Sciences, Iran
D
uring the recent years, there has been rising attention to the development of topical delivery systems to facilitate drug
permeation through the skin.The drugs commonly used are those with debatable oral administration. Although piroxicam
is a valuable anti-inflammatory, antipyretic and analgesic drug, long term oral administration is limited due to the various GI
side effects. The main aim of this study was to prepare and assess a topical formulation of piroxicam based on Solid Lipid
Nanoparticles (SLNs), to improve its percutaneous permeation rate. Topical nano-lipidic gel of piroxicamwas formulated and its
pharmaceutical characteristics were evaluated. Piroxicam loaded SLNs were formulated by solvent emulsification evaporation
method. The SLNs were composed of stearic acid and cholesterol as lipid phase, Brij35 and Brij72 as a stabilizer and acetone was
used to dissolve the lipidic ingredients of the formulation. Particle size assessment, drug loading determination, entrapment
efficiency assessment, and
in vitro
release study and skin permeation of the piroxicam was determined to characterize the SLNs
and then these nanoparticles were formulated in gel as topical delivery system to assess percutaneous permeation of piroxicam.
The SLNs were prepared in different size ranges from 100-300 nm and drug release behavior from two different nano-sized
SLN suspensions was evaluated. Piroxicam nano-lipidic gel showed increased skin permeation of the drug over commercial
piroxicam gel formulation and also mean particle size of formulated SLNs had significant effect on permeation rates.
smsamani@sums.ac.irAmerican Journal of Ethnomedicine, 4:2
DOI: 10.21767/2348-9502-C1-003