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Page 23
Volume 05
Journal of Infectious Diseases and Treatment
ISSN: 2472-1093
JOINT EVENT
Applied Microbiology-2019 & Antibiotics 2019
Immunology 2019
October 21-22, 2019
October 21-22, 2019 Rome, Italy
&
&
8
th
Edition of International Conference on
Antibiotics, Antimicrobials & Resistance
12
th
International Conference on
Allergy & Immunology
6
th
World Congress and Expo on
Applied Microbiology
Plantaricin plays a role for post-fermented tea
Yolani Syaputri
1
, Masanori Horie
2
and
Hitoshi Iwahashi
1
1
Gifu University, Japan
2
Health Research Institute, AIST, Japan
P
ost-fermented tea samples, before fermentation, primary and secondary fermentation process fromeach company
were collected and studied. Klebsiella
pneumoniae subsp. ozaenae, Pseudomonas glareae, Klebsiella variicola
, and
Pseudomonas aeruginosa were observed in tea leaf before fermentation. Furthermore, in primary and secondary
fermentation
Lactobacillus plantarum
became one of the major lactic acid bacteria species can survive in these
processes. The results showed that
plnA, plnEF
, and
plnNJK
existed in primary and secondary fermentation. Two
potentials
L. plantarum
were isolated from post-fermented tea samples,
L. plantarum
IYO1501 and
L. plantarum
IYO1511. plnA and plnEF were encoded on plasmid of
L. plantarum
IYO1501 and
L. plantarum
IYO1511. Whereas,
plnNJK was encoded on
L. plantarum
IYO1511 chromosome. Schematic representation of
plnA, plnEF
, and plnNJK
gene cluster in
L. plantarum
showed different schematics gene cluster. The cluster structure of plantaricin encoding
region was not shown yet around plantaricin gene region before. plnA on
L. plantarum
IYO1501 showed 96%
homology of GenBank. The results showed clearly that
L. plantarum
IYO1501 and
L. plantarum
IYO1511 produced
plantaricin on plasmid and on chromosome and play an important role for the success of the product.
Recent Publications
1.
Strong Antimicrobial of Lactic Acid Bacteria and Species Identification of Virgin Coconut Oil Products
in Padang West Sumatera, Indonesia (2017). International Journal on Advance Science, Engineering and
Information Technology (IJASEIT)
2.
Isolation and Characterization of
Lactobacillus spp
. From Virgin Coconut Oil, West Sumatra. 2018.
International Symposium on Animal Production and Conservation for Sustainable Development
3.
Probiotic Research in Several Products of Virgin Coconut Oil from Padang, Indonesia. 2018. Journal of
Physic
Biography
Yolani Syaputri is pursuing her PhD at Gifu University. She has worked in the area of especially food fermentation, lactic acid bacteria, and bacteriocins. Currently,
she is developing fermented tea from Gifu, Japan. She is interested to find a potential lactic acid bacterium which is isolated frommany samples. She focused on
identification bacteriocin gene or structural gene, immunity protein and secretion gene to get the genetic information and develop them to make a good product.
Yolani Syaputri et al., J Infec Dis Treat 2019, Volume: 05
Figure: plnEF, Gene cluster L. plantarum
IYO1501 on plasmid