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Volume 05
Journal of Infectious Diseases and Treatment
ISSN: 2472-1093
JOINT EVENT
Applied Microbiology-2019 & Antibiotics 2019
Immunology 2019
October 21-22, 2019
October 21-22, 2019 Rome, Italy
&
&
8
th
Edition of International Conference on
Antibiotics, Antimicrobials & Resistance
12
th
International Conference on
Allergy & Immunology
6
th
World Congress and Expo on
Applied Microbiology
Retail chicken carcasses as a reservoir of antimicrobial-resistant
Escherichia coli
Nahla Omer Eltai, Hadi Yassine, Tahra El- Obied, Sara Al hadidi Asma Al Thani
and
Walid Alali
Biomedical Research Centre-Qatar University, Qatar
A
ntibiotic resistance is one of the major public health challenges in both developed and developing countries.
The development and spread of resistant bacteria have been linked to the misuse of antibiotics in human
and animal populations. Moreover, the spread of resistance has been associated with the consumption of food
contaminated with pathogenic and non-pathogenic resistant bacteria. There is limited information of antibiotic
resistance contamination of raw chicken meat in Qatar. The objective of this study was to determine the prevalence
of antibiotic resistance among local and imported chicken whole carcasses in Qatar. A total of 270 chicken carcasses
locally-produced (chilled) and imported (chilled and frozen) were obtained from three different hypermarket stores
in Qatar. A total of 216 Escherichia coli (E. coli) were isolated and subjected to antibiotic susceptibility testing
using disc diffusion method. Furthermore, extended-spectrum β-lactamase (ESBL) production was determined via
double disc synergetic test. In addition, isolates harboring colistin resistant was determined using multiplex-PCR
and DNA sequencing. Nearly 89% (192/216) of the isolates were resistant to at least one of the 18 antibiotics. In
general, isolates from local and imported chicken carcasses showed relatively higher resistance to sulfamethoxazole
(62%), tetracycline (59.7%), ampicillin and trimethoprim (52.3%), ciprofloxacin (47.7%), cephalothin, colistin
(31.9%) and gentamicin (15.7%). On the other hand, less resistance was recorded against amoxicillin/clavulanic acid
(6%), cefuroxime (6.9%), ceftriaxone (5.1%), nitrofurantoin (4.2%) and piperacillin/tazobactam (4.2%), cefepime
(2.3%), meropenem (1.4%), ertapenem (0.9%), fosfomycin (1.8%), and amikacin (0.9%). Nine isolates (4.2%) were
ESBL producers. Furthermore, 63.4% (137/216) were multidrug resistant (MDR). The percentage of MDR, ESBL
producers, and colistin-resistant isolates were significantly higher among local chilled isolates compared to imported
chilled and frozen chicken samples. Our findings indicate high antibiotic resistant prevalence in chicken carcasses
in Qatar, including ESBL, MDR and colistin resistance. Such resistant E. coli could potentially spread to humans
through consumption of chicken meat. Development and implementation of a stewardship program to control and
monitor the use of antibiotics in animal production locally and introduce testing of imported chicken for antibiotic
resistance are needed in Qatar.
Biography
Nahla Omer Eltai has completed her PhD at Humboldt University, Berlin, Germany; Postdoctoral studies from University of the West of England, U K She is a
Research Associate at Biomedical Research Centre, Qatar University, Qatar. She has published more than seven papers in the field of antibiotic resistance in
reputed journals. Her research interests are multidisciplinary with emphasis on molecular diagnostic approaches, antimicrobial susceptibility and resistance, test
of new natural antimicrobial agents. She is adopting the one health system approach by studding antimicrobial resistance in agriculture, environment and human.
Nahla Omer Eltai et al., J Infec Dis Treat 2019, Volume: 05