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Page 18
Volume 05
Journal of Infectious Diseases and Treatment
ISSN: 2472-1093
JOINT EVENT
Applied Microbiology-2019 & Antibiotics 2019
Immunology 2019
October 21-22, 2019
October 21-22, 2019 Rome, Italy
&
&
8
th
Edition of International Conference on
Antibiotics, Antimicrobials & Resistance
12
th
International Conference on
Allergy & Immunology
6
th
World Congress and Expo on
Applied Microbiology
Hospital-acquired infections in Ukraine
Yulian Konechnyi, Andrij Albokrinov
and
Olena Korniychuk
Danylo Halytsky Lviv National Medical University, Ukraine
Introduction
: According to WHO, approximately 15.0% of all hospitalized patients suffer from Hospital-Acquired
Infection (HAIs). Data from USA and Europe show 1,3% to 2,03% cases of HAIs in hospitalized patients. In
Ukraine -5-7.000 proven cases of HAIs and 500.000 unregistered cases per year. One of the methods to prevent HAIs
is control strategy in Intensive care unit (ICU), where last-line antibiotics are currently used.
Objective
: To define spectrum of microbial flora and antimicrobial sensitivity of HAIs in some ICU in Lviv city,
Ukraine.
Methods
: It was investigated 124 clinical materials from 60 patients at the public hospitals in Lviv during
July 2018 - May 2019. Were isolated 144 strains of pathogenes. HAIs was defined as infection that appeared in
patient later than 48 hours after hospital admission. Causative bacterial strains were isolated and identified
using bacteriological method (Mueller Hinton Agar, Blood Agar, Saburo Agar, selective & differential media
CHROMID*VRE, CHROMID*MRSA SMART, CHROMID*Candida etc. (Biomerieux)) and biochemical tests
MIKRO-LA-TEST NEFERMtest24, CANDIDAtest21, STAPHYtest24 etc. (ErbaLacema). Antibiotic sensitivity test
was done according to Kirby-Bauer technique.
Results
: HAIs structure was as follows:
Staphylpcoccus lentus
(17.7%),
Eschrichia coli
(20.2%),
Klebsiella pneumoniae
(9,7%),
Pseudomonas aeruginosa
(24.2%),
Streptococcus spp
. (2.4%),
MRSA
(3,2%),
Candida albicans
(8.1%).
There were several types of HAIs which are rarely founded, such as
Staph.simulans, Staphylococcus gallinarum
,
Staphylococcus haemolyticus Staphylococcus lugdunensis, Aerococcus viridans
,
Pragia fontium, Aeromonas ichthiosmia,
Raoultella terrigena, Burkholderia pseudomallei, Macrococcus caseolyticus, Pseudomonas oryzihabitans, Pseudomonas
putida, Candida dubliniensis, Candida membranifaciens, Candida glabrata
. Anti-microbial sensitivity assessment
showed that more than 90.0 % pathogens were multidrug resistance (MDR). 75% of strains were atypical strains
(T-index ≥ 0,25).
Conclusion
: In the era of antibiotics HAIs are still uncontrollable. Most cases of HAIs in Ukraine are not recognized
as epidemics. More than 90.0 % pathogens are MDR and most of them are atypical strains. Diagnostic capabilities
show a number of rare species of microorganisms that cause nosocomial infections. It is important to use this fact
when one attempts to design prevention or control strategy to reduce HAIs either at WHO level, or at the level of
local health care unit.
Acknowledgement
: We thank directors of the hospitals and ICU teams.
Ethical Committee
: 25/06/2018 № 6.
Biography
Yulian Konechnyi is doing his PhD in microbiology at Danylo Halytsky Lviv National Medical University, Ukraine. He has completed his MD and have specialization
in internal medicine. He has worked as lecturer at the department of microbioly in microbiology, virusology and immunology topics, at Danylo Halytsky Lviv
National Medical University, Ukraine. He has published 10 different papers.
Yulian Konechnyi et al., J Infec Dis Treat 2019, Volume: 05