Using recurrent breeding selection and intermating are useful for cotton breeding programs, however some best lines maybe include some unfavorable alleles this require to make new crosses and also require hybridization in early segregating, generation. This work was carried out in the Agronomy Department, Faculty of Agriculture, Kafr Elsheikh University and Sakha Agriculture Research Station, Kafr Elsheikh, Agricultural Research Center, Egypt, during 2015 to 2018 growing seasons. The main objective of this investigation was to study the efficiency of biparental progenies derived from inter population random mating in F2 generation (North Carolina Design-1, six plants as male and nine plants as female) of the cross between cotton varieties (Giza45 × Giza94). In 2016 growing season, the resulted 54 crosses were evaluated as individual plants with the original parents, and 25 plants were resulted from inter and intra crosses selection in the end of season. In 2017 growing season the 25 families were evaluated with parents and subjected to self-pollination as biparental selfed pollinated (BIPs1), in the end of season 27 plants were resulted from inter and intra crosses selection. In 2018 growing season the 27 families were evaluated with parents and subjected to self-pollination as biparental selfed pollinated (BIPs2). All genotypes were evaluated for agronomic and fiber traits. The results for cotton of BIPs1 and BIPs2 families in dictated significant differences for most of the families compared with better parent were higher mean values for boll maturation period, boll weight, seed cotton yield / plant, earliness index, lint yield / plant, seed index in BIPs1 respectively. The genotypic variation coefficient GCV of all traits in (BIPS2) families was greater than (BIPS1) families except earliness index, seed cotton yield, lint yield per plant and fiber fineness. Genotypic correlation values for biparental (BIPS1) and biparental (BIPS2) were some relationships among traits in (BIPS1) changed by using intermating, the correlation value between seed cotton yield and boll weight, lint percentage, seed index, lint index and micronaire changed from non-significant to highly significant correlation. Therefore, continuous and evaluation to selection for these BIPS depends on performance to families may be useful to get better prospects for deriving genotypes with desired traits.