Synthesis of magnetic nanoparticles coated with palmitic acid and folic acid and study their interaction with prolactin

In the present work, two coated magnetic nanoparticles (MNPs) were prepared using co-precipitation method of Fe3O4 followed by reaction of these MNPs with either folic acid or palmitic acid under optimum condition to produce coated MNPs called MNP@Folic and MNP@Palmitic, respectively. The synthesized coated MNPs were characterized and visualized by different advanced techniques. The interaction between the prepared MNPs and prolactin hormone was studied as a potential method for the extraction of PRL from biological fluids such as blood or for the immobilization of PRL on the surfaces of MNPs as a new application of these insoluble particles. MNPs have the ability to extract suitable amounts of PRL from solution. The differences between the quantities adsorbed from MNPS to another were little. Adsorption isotherms have S-shape on Giles classification for the adsorption isotherms. Freundlich's equation was applied for all the interaction processes indicating the heterogeneity of the forces on the surfaces that the PRL bound to. Desorption experiments showed very low desorption quantities indicating the magnitude of the forces that immobilize the PRL molecules on the surfaces of the prepared MNPs.

Author(s): Hussain Kadhem Al-Hakeim,Mea'ad Mohammed Redha and Ashour Hamood Dawood

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