Scytosiphon lomentaria and Padina pavonica are the two members of the Phaeophyceae. Our study has reported that these two brown macroalgae exhibit the important biological potentials such as antioxidant, hypoglycemic and hypolipidemic activities. However, S. lomentaria exhibit potent antioxidant activities, it has been observed that P. pavonica has strong antioxidant potentials such as 251.56 ± 1.33 mg GA g-1 dw total phenolic content, 70.50 ± 1.4% DPPH radical scavenging activity and 60.6 ± 1.6% bleaching activity. In addition to antioxidant potentials, the species have not displayed significant cytotoxicity at the administered doses on MCF-7. Nevertheless, the highest dose for P. pavonica (100 µg ml-1) caused to moderate cell viability inhibition (43%). A meaningful IC50 value could not be calculated for S. lomentaria. Many enzyme inhibition tests are commonly used as indicator for biological activities. Both P. pavonica and S. lomentaria have higher α- glucosidase enzyme inhibition potential than acarbose. The strong inhibition percentages of these cold methanol extracts (89 and 88%, respectively) indicate their hypoglycemic activity. The pancreatic lipase enzyme inhibition assay was used to evaluate the hypolipidemic potentials of these microalgae. P. pavonica have strongly inhibited this enzyme when compared to orlistate (93 and 95%, respectively). These results have figured out the medical and pharmaceutical potentials of these species.