Expression Analysis of Hsp60 Gene from Albino Northern Snakehead (Channa argus) after Bacterial Infections

The great albino northern snakehead, Channa argus, one of the most important economical fish in China. In present study, the expression changes of AcaHSP60 gene and Acahsp60 protein in spleen, liver, kidney, and brain of albino C. argus were detected and analyzed after infected with Edwardsiella tarda (strain NO. DL1476) at different time points. Quantitative real-time PCR (qRT-PCR) and western blot analysis revealed tissue-specific AcaHSP60 and Acahsp60 expression in the control group. AcaHSP60 mRNA levels were highly significantly increased and reached maximum in the spleen (48 h post injection), kidney (48 h post injection), and brain (24 h post injection), while it was kept at a high level and had significantly decreased in the liver post injection. These results showed that AcaHSP60 expression was significantly tissue-specific and might be sensitive to pathogen infection in albino C. argus.

Author(s): Aiguo Zhou1, Zhuolin Sun, Shulin Liu, Shaolin Xie, Zhenlu Wang, Yue Zhang, Yanfeng Chen and Jixing Zou

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