Production and Optimization of Pectinase from Bacillus sp. MFW7 using Cassava Waste

This study investigated pectinase production and optimization by bacterial strain MFW7 isolated from fruit market wastes. Initial screening process identified the best pectinolytic strain and it was characterized by 16S rDNA sequencing and designated as Bacillus sp. MFW7. Optimization of process parameters were carried out by altering the physico-chemical environment of the production medium. High pectinase production was observed at 72 h of incubation at 35°C with the initial pH of 6.5. Various nutrients were investigated in terms of their effect on the production of extracellular pectinase using Cassava waste as substrate. Lactose in combination with peptone supported maximum pectinase production. Purified pectinase showed a molecular weight of 37 kDa observed by means of SDS-PAGE.

Author(s): Mukesh kumar D J*, Saranya G M, Suresh K, Andal Priyadharshini D, Rajakumar R and Kalaichelvan PT

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