Isolation, characterization and identification of endophytic bacteria by 16S rRNA partial sequencing technique from roots and leaves of Prosopis cineraria plant

Plant roots are potential hosts to a plethora of beneficial microorganisms including mycorrhizal fungi, rhizobia bacteria, antagonistic yeasts and endophytic fungi. Endophytic bacteria live symbiotically with the plant and in turn helping the plant in number of ways. The present investigations were undertaken to isolate and identify bacterial endophytes in root and leaf tissue of Prosopis cineraria plant growing at Pune, India. A total of two endophytic bacteria were isolated from the parts of the plant .On the basis of the morphological and biochemical characterization of the endophytes as well as 16S rRNA sequencing technique they are identified as Bacillus subtilis and Stenotrophomonas maltophilia. Both the isolates Bacillus subtilis & Stenotrophomonas maltophilia were present in root tissue while only one i.e. Stenotrophomonas maltophilia isolated from leaf tissue. Studies on the optimization of growth of the isolates were performed by varying pH & temperature conditions. The ability of bacterial isolates were tested for different enzyme activity and also screened for sensitivity of different antibiotics.

Author(s): Rekha M. Gupta, Prathamesh S. Kale, Madhuri L. Rathi and Nikhil N. Jadhav

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