In-vivo and in-vitro comparative study of primary metabolites and antioxidant activity of Catharanthus roseus

The present study describes a comparative study of primary metabolites and antioxidant activity. Various tissues including leaf derived callus of Catharanthus roseus. Fast growing callus was obtainable through leaf explants on MS media supplemented with 2, 4-D 1mg/l. The calluses were fragile and yellowish in colour. Maximum soluble sugars, starch, protein and lipid found in leaf, maximum amount of phenolic contents were found in stem. Leaf extract showed maximum superoxide radical scavenging activity (37.02±1.14%) than other plant parts while root extract have maximum 2,2- diphenyl-1-picryhydrazyl (DPPH) free radical scavenging activity (72.39±1.28%).

Author(s): Anshul K. Jain, Meena and Dinesh Kumar

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