Abstract

Hydroxy radical and DPPH scavenging activity of crude protein extract of Leucas linifolia: A folk medicinal plant

Leucas linifolia belongs to the family Lamiaceae and is a well know traditional medicinal plant in India. It is an herbaceous annual weed. In ayurveda, it is used for the treatment of cough, cold, fever, loss of appetite, skin disease, head ache, jaundice, Snake bite and scorpion sting. It has shown hepato protective activity against dgalactosamine and CCl4 induced hepatotoxicity in rats. Methanol extract of the plant has shown wound healing property. Antibacterial, antipyretic and antitussive activity of the plant have been reported which confirms its traditional medicinal usage from the time of ayurveda. In the present study, we have shown the antioxidant activity of the ammonium sulphate precipitated protein extract of Leucas linifolia. Crude protein extract of Leucas linifolia (CPLL) extract showed In vitro antioxidant activity in both hydroxyl radical scavenging and as well as in DPPH scavenging activity in Dose dependant manner . At 500μg/ml concentration of CPLL, it showed 78 and 63% inhibition, with an IC50 value of 150 and 175μg/ml in hydroxyl radical and DPPH assay respectively. As CPLL extract, scavenge hydroxyl radical more than the DPPH radical, it might have DNA protectant activity also. To conclude, CPLL showed a good antioxidant activity and might be an alternate to synthetic antioxidants available in the market.


Author(s): Harsha Ramakrishna, Sushma S. Murthy, Divya R, MamathaRani D.R and Panduranga Murthy G*

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