The aim of the present study was to assess the in vitro antiglycation activity of aqueous extracts of some traditional antidiabetic plants by determining the degree of non-enzymatic hemoglobin glycosylation. In the present investigation, the best concentration and time to incubate glucose with hemoglobin was determined. Thereafter, the glycosylation degree of hemoglobin in the presence of plant extracts at different concentrations and in their absence was measured colorimetrically at 520nm.The inhibitory effect on glycosylation of hemoglobin at the four concentrations viz. 250 μg/ml, 500 μg/ml, 750μg/ml and 1000 μg/ml was estimated as follows: for M. fragrans 17.02%, 26.19%, 34.30% and 42.75%, T. indica; 31.77%, 41.14%, 51.63% and 60.20 %, C. bonducella; 44.01%, 60.13%, 69.70%, 76.33% respectively. The results of the study clearly highlighted that non-enzymatic nature of hemoglobin glycosylation could be effectively inhibited by the extracts of C. bonducella, T. indica and M. fragrans at a desirable concentration.