In the present study, seed crude extracts of Solanum trilobatum was studied for its ability to inhibit the growth of dental (bacterial) pathogens (Staphylococcus aureus, Streptococcus mutans, Streptococcus salivarius, Streptococcus sanguinis and Lactobacillus acidophilus). Collected seeds of S. trilobatum were properly washed and shade dried at room temperature, crushed and extracted in acetone, petroleum ether and chloroform. The antibacterial activity of extracts was examined by ager well diffusion method at 200 mg mL-1sample concentration. Phytochemical analysis was done for plant extracts. The results of anti-bacterial activity were found that acetone extract of S. trilobatum was most effective against all tested bacterial pathogens. Maximum anti-bacterial activity was observed against S. salivarius (23 mm)and lowest activity against S. sanguinis(9 mm). The phytochemical analysis revealed the presence of alkaloids, flavonoids, steroids, terpenoids, tannins and saponins which might be accountable for its anti-bacterial potentiality. The results validate the traditional uses of S. trilobatum in treatment of dental diseases.