Random amplified polymorphic DNA (RAPD) analysis for genetic diversity in Terminalia bellirica species (Roxb.), an important medicinal tree

Terminalia bellirica Roxb. (family Combretaceae) also known as Bahera in hindi, is one of the important constituents of India preparation Triphala. It is found in all deciduous forest of India, the present study was formulated to acess the genetic diversity within each population, among the populations and also in micro propagated plantlets of T.bellirica using random amplification amplified polymorphic DNA (RAPD) marker. Plants were collected from four different provenances of Chhattisgarh State, India, and ten from in vitro produced plantlets. Twenty primers were used for RAPD analysis. RAPD analysis yielded 168 total bands of which 152 polymorphic bands (90.0 percent polymorphism), with an average of 8.4 amplified bands per primer. The dendrogram was constructed using UPGMA method. Our results reveal very high level of genetic diversity in the species. These results also indicate that RAPD is a good molecular marker to study the genetic diversity of these species.

Author(s): Sahu Bharti* and Koche Vijaya

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