Quantitative assessment of genomic DNA isolated from different populations of Commiphora wightii (Arnott.) Bhandari, in Rajasthan

Commiphora wightii commonly known as “Guggal” is found in arid and semi-arid regions including the deserts of India, Africa and Pakistan. The present study was aimed to investigate the quantity as well of quality of genomic DNA extracted from the different wild populations present in the state of Rajasthan. Samples were collected from different eco climatic regions in the state. An optimized protocol was used for the extraction of genomic DNA of the species, owing to the high content of polysaccharides and phenols present in it. The use of 3M NaCl proved to be especially beneficial during the extraction process and helped yield pure and high quality DNA samples that were verified through the ISSR profiles generated and were deemed fit for research downstream. The study also revealed that diverse edaphic and climatic conditions have had no major effect on the quantity and quality of genomic DNA of C.wightii.

Author(s): Priyanka Vyas and Ramesh Joshi*

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