Phytochemical screening and haemolytic activities of crude and purified saponins of aqueous and methanolic extracts of leaves of Tephrosia vogelii Hook. F

The phytochemical screening of the aqueous and methanolic extracts of leaves of Tephrosia vogelii Hook.f. was carried out to ascertain the presence of saponins and further studies carried out on the crude and purified saponins. The results of phytochemical screening using aqueous(with a yield of 16.396%) and methanol (with a yield of 12.316%) extracts revealed the presence of cardiac glycosides, alkaloids, saponins and phlobatannins. However, the plant was devoid of anthraquinones and tannins. The presence of saponins was further detected by the haemolysis of red blood cells. Crude PBS extract possessed haemolytic activities against human erythrocytes (A,B,O and AB) but at varying degrees. The haemolytic activities for full and partial haemolysis(FH and PH) with human red blood cells A, B, O and AB ranged between 20to 24, 24, 26 and 24 respectively. The haemolytic activity of the purified saponins(PS) was also determined. The results of the haemolytic activity of crude aqueous extract and purified saponins using erythrocytes of blood group O were the same (PS321 FH, 23PH) and (PS521 FH, 23 PH).

Author(s): Inalegwu B and Sodipo O. A

Abstract | PDF

Share This Article