To determine the antibacterial, antifungal and phytochemical properties of petroleum ether, ethyl acetate, chloroform, methanol extracts and to evaluate the bioactive components from leaves of Justicia adhatoda (Linn). The extracts were tested against bacteria and fungus through disc diffusion assay. The phytochemical compound screened by qualitative and GC-MS method. Qualitatively analyzed as alkaloids, anthraquinones, flavonoids, saponins, phytosterols, triterpenoids and polyphenols gave positive results. Carbohydrates and cardio glycosides gave negative results. In the GC-MS analysis 9 major bioactive components were identified in the Petroleum ether extract of Justicia adhatoda. The four crude extracts of Justicia Adhatoda where investigated for their potential anti-bacterial and anti-fungal activities. All extracts showed anti-bacterial and anti-fungal activities against all the organisms tested except Pseudomonas aeruginosa and Proteus vulgaris. This study provides scientific evidence on the traditional use of Justicia Adhatoda leaf extract in treating microbial diseases. Further, the leaf extract can possibly be used to produce alternative forms of antimicrobials.