Many diseases are associated with oxidative stress caused by free radical. Plants are rich in variety of bioactive compounds in the form of primary metabolites and secondary metabolites including aromatic substance, most of which are phenols or their oxygen substituted derivative (such as tannin). These compounds possess high antioxidant properties. Spices have been known not only for flavouring, colorant and preservative but also being used for their potential benefit. In the present study, Zanthoxylum armatum, Allium odorum and Eryngium foetedum are taken into consideration to investigate the phytochemical constituent and antibacterial properties in their ethanol and aqueous extract. The result showed the presence of alkaloids, tannins, saponins, phenols, flavonoids and volatile oil that are common to all the ethanol extract. The antimicrobial activity of these solvent extract was test against two gram positive bacteria (Staphylococcus aureus: ATCC-11632 and Bacillus cereus :ATCC-10876) and two gram negative bacteria (Klebsiella pneumonia: ATCC-10031 and Pseudomonas aurogenosa :ATCC- 15442) by agar well diffusion method. In both ethanol and aqueous extract of Zanthoxylum armatum showed maximum antibacterial activity.