Induction of vanillin related compounds from nodal explants of Vanilla planifolia using BAP and Kinetin

The multiple shoots of Vanilla plantifolia obtained from the nodal explant on MS medium supplemented with 6- benzylaminopurine (BAP) 2 mg l-1 in combination of α-Napthalene acetic acid (NAA) 1mg l-1 and kinetin 20 μg g-1 f. wt of cells were maintained by regular sub culturing every 30 days and also cultured liquid MS medium of the same combination growth regulators . Cell suspension culture of Vanilla plantifolia was used for studies on the biosynthesis of vanillic acid. Maximal induction was observed at a kinetin concentration of 20 μg g-1 fresh weight of cells. Cell cultures of V. plantifolia were capable of producing benzoate derivatives and enzymes involved in the synthesis appear to be present within the cells. Kinetin was used as an elicitor to induce vanillic acid formation. The induction of the few enzymes of the phenypropanoid pathway may result in the formation of vanillic acid under cell suspension cultured conditions. In vitro cultures were extracted with ethylacetate and the amount of aromatic compounds were determined in explants and compared with different developmental stages of explants were analyzed using HPLC for the presence of vanillin flavor and related compounds. This system can be used successfully to understand the biosynthesis of vanilla flavor compounds in organized shoot cultures and compared with normal plants. Explants cultured for 12 to15 weeks in MS + BAP +NAA+ Kinetin medium had attained the stage of vigorous proliferation, which were transferred for further mutiplication then plantlets were exposed to natural condition for primary hardening.

Author(s): Renuga G.* and Saravana Kumar S. N

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