In vitro direct organogenesis in roots of Ipomoea batatas

In vitro culture is currently used to produce plant material for micropropagation, genetic manipulation and ex situ conservation of commercial species. The organogenic potential of root explants derived from cultured both apical and lateral buds of cuttings of Ipomoea batatas (sweet potato) cv. UNPRG-358 and cv. IN-180 was investigated in response to different incubation conditions such as gelled and liquid culture medium, size of explants (2.0 and 5.0 cm in length with and without apex), type of vessels, different root portions (proximal, medial and distal) and the cytokinins BAP (6-benzyladenine), ZEA (Zeatin) and 2iP (Isopentenyl adenine). The most adventitious buds developed from explants cultured in liquid media under a 16-h photoperiod, and the use of BAP and ZEA promoted the development of more adventitious buds than 2iP. Organogenesis in I. batatas occurred via a direct pathway, which was confirmed by anatomical studies. These histological analyses showed that adventitious buds originated directly from the proliferation of cortex cells, and also at the base of the lateral root formation, and that the lateral roots were regenerated directly from the proliferation of perycicle cells opposite the poles of the primary xylem.

Author(s): Consuelo Rojas Idrogo, Jorge Chanamé-Céspedes, Eny IS Floh, Walter Handro and Guillermo E Delgado-Paredes*

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