In vitro callus induction and plant regeneration potentiality were studied from mature embryo of three Indian rice (Oryza sativa L.) varieties. Study was done by using callus induction medium (Murashige and Skoog, 1962) having different concentration of 2, 4-D viz., 1.0, 1.5, 2.0, 2.5 mg/l. All the three varieties exhibit highest frequency of callus induction at 2 mg/l 2,4-D. The callus induction frequency varied from 63.36% to 92.23%. Sita had maximum frequency of callus formation only, while Masuri showed embryogenic organogenesis (shoot formation) followed by the formation of callus and Rupali showed increase in multi root development in the early stages of callusing. Plant regeneration efficiency of in vitro grown plantlets were further observed and successfully transplanted into soil condition.