Biophytum Sensitivum Linn., leaves and stems were extracted with Petroleum ether, Ethyl acetate and Methanol by successive hot extraction. All the extracts were evaluated for Anthelmintic activity with Indian earthworm (Pheretima posthuma). Leaves ethyl acetate extract has shown significant activity for concentration 25mg/ml, 50 mg/ml, 100mg/ml with paralysis time 85.0±1.9, 66.0±1.3 40.8±1.0 and Death time 91.3±1.1, 81.2±1.7, 50.5±0.8 respectively. The anthemintic activity may be shown due to biflavonoids and tannins present in ethyl acetate extract. Other extracts also shows comparatively low activity.