Acalypha indica distributed in the southern part of India particularly in Tamilnadu and it has potential medicinal properties and used as diuretic, anthelmintic and for respiratory problems such as bronchitis, asthma and pneumonia. The present work has been designed to investigate the preliminary phytochemical, antibacterial and GC-MS analysis of methanolic extract of the plant. Phytochemical screening of leaves extract revealed that the presence of alkaloids, tannins, steroids, saponins, flavanoids, glycosides and phenolic compounds. The methanolic extract of leaves is found to be exhibit activity against Escherichia coli, Salmonella typhi, Pseudomonous aeruginosa and Staphylococcus aureus. Various phytochemical compounds are identified by GC-MS analysis.