Comparison studies on callus induction of fresh and reused explants of Atropa belladonna

Atropa belladonna belongs to the family Solanaceae. It is the most poisonous plant of western Hemisphere. All parts of the plant contain tropane alkaloids. The active agents of belladonna are atropine, hyoscine and hyoscyamine. Belladonna has been used in traditional treatments for centuries for an assortment of conditions including headache, pectic ulcer, histaminic reactions, motion sickness etc. Micropropagation of ancient medicinal plant is necessary to produce many such secondary products. Micropropagation of Atropa belladonna at higher temperature had showed differentiated results. 25±3°C incubation for 16h light/8h dark showed many altering results with different hormonal concentration. Fresh explants and reused explants (intact and healthy explants transferred from hormone free media to new media), when transferred to MS media with NAA and Kinetin resulted in altered callus formation. Growth rate was varied, recorded and callus dry weight calculated for comparison study between fresh and reused explants.

Author(s): Asha Rani N.S and Prasad M.P*

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