Callus induction and plantlets regeneration of two rice (Oryza sativa L.) genotypes under saline stress conditions

The present investigation was aimed to obtain salt tolerant genotypes of Inti and Viflor rice cultivars. In this study, callus induction and plant regeneration responsiveness were tested using different concentrations of 0.0-2.0% (w/v) NaCl, for callus induction, and 0.0-1.0% (w/v) NaCl, for plant regeneration, which added in MS based media. Marked variation was observed both in calli proliferation and plant regeneration among the two rice cultivars. Among the cultivars tested, cv. Inti and cv. Viflor produced the highest percentage (91% and 80%, respectively) of callus with size (+) on the MS medium supplemented with 0.75% and 1.0% NaCl, respectively. Plant regeneration of both cv. Inti and cv. Viflor was 62.5% and 75%, respectively, at 0.5% NaCl, but decreased to 12.5% at 1.0% NaCl. The results of the present study showed the decreasing trend in callus induction and plant regeneration with increasing concentrations of NaCl.

Author(s): Grimanesa M. Mori-Gastelo, Consuelo Rojas-Idrogo, Jorge Chanamé-Céspedes and Guillermo E. Delgado-Paredes*

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