The experiment was carried out to study the genetic diversity of indigenous Jujube germplasm available in Dumki Upazilla, Patuakhali, Bangladesh during the period from January 2016, to February, 2017. 42 jujube germplasm were selected from different homesteads and their passport data were prepared according to GPS location. Significant variations were recorded among the accessions in respect of different parameters. Among the germplasm, D9 (22.5 g) and D1 (21.95 g) showed the highest performance in weight of fruits followed by D30, D35, D42. The most desired character pulp with peel weight, D9 (21.5 g) gave the highest result followed by D30 (16.95 g), D35 (16.44 g) where lowest was found in D12 (2.86 g). In context of seed weight and leaf area, highest seed weight was found in D38 (3.07 g) and leaf area in D17 (31.4 g). The maximum TSS (3.08) and TA (3.97) percentages were recorded in D23 jujube germplasm where minimum in D38 (0.53 and 0.38 respectively). Among the germplasm D31, D42 and D12 denote the significant results in the properties of vitamin C (37.58), TSS/TA (2.6) and pH (4.71) contents respectively. Cluster analysis based on morphological and biochemical traits grouped the genotypes into five classes. Regarding intercluster distance for biochemical traits, cluster II showed maximum genetic distance (29.83) from cluster III which suggesting wide diversity where lowest value was recorded between cluster I and IV (5.82). The highest cluster means for TSS (2.48), TA (3.05) and Vitamin C (37.45) were obtained from cluster II. For morphological traits, cluster I showed maximum genetic distance (25.72) from cluster V and the highest cluster means for fruit weight (21.95g), seed weight (1.18 g), pulp with peel weight (20.77 g) and fruit diameter (3.02 cm) were obtained. These results showed that D42, D9, D30, D35 were suitable for raw consumption as table fruit and D18 was suitable for pickle preparation.