The present study deals with the phytochemical investigation and therapeutic use importance of two plants Andrographis paniculata and Aloe vera. It is an important medicinal plant worldwide trend towards the utilization of natural plant remedies has created an enormous need for the use of medicinal plants. The methanol extract of same plants were subjected to analysis the antimicrobial activity by disc method. The extracts of the plant leaves showed antimicrobial activity against both gram(+) and gram(-) bacteria, Maximal antibacterial activity was observed against streptococcus faecalis. The maximal antimicrobial activity was against Diameter of zone inhibition exhibited by Andrographis paniculata and Aloe vera methanol extract in various concentrations.