Achyranthes aspera Linn. (A.aspera) is an annual, stiff erect herb, about 0.3 to 0.9m high and found commonly as a weed throughout India. Diabetes mellitus is a chronic metabolic disease with life-threatening complications. Despite the enormous progress in conventional medicine and pharmaceutical industry, herbal-based medicines are still a common practice for the treatment of diabetes. This paper explains evidence based-information regarding the antidiabetic activity of ethanol extract of Achyranthes aspera with alloxanised mice for the estimation of level of glucose and cholesterol. In case of alloxanized mice due to administration of ethanol extract of A.aspera the level of blood glucose was found to be increased by 123% and 128% on the fifteenth and thirtieth day of exposure respectively. It was obvious from the result that, the ethanol extract of A.aspera played a beneficial role in the maintaintence of glucose level in alloxanised mice. Due to administration of lone effect of ethanol extract of A.aspera the level of cholesterol was found to be decreased in mice by 3.6% on fifteenth day of exposure. However, after completion of exposure period the tendency to decrease in the state of hypocholesterolemia was pronounced and was found to be 5.5%. From the results of the present study it is obvious that the A. aspera has potent anti diabetic effect in alloxan induced diabetic mice and could therefore be used as a remedy for the treatment of diabetes mellitus.