The objective of the present study was to evaluate the phytochemical constituents, anti-oxidant activity and highperformance liquid chromatography (HPLC) analysis of glycoside compound from apple and avocado fruit methanolic extract. Preliminary screening involved the qualitative methods to detect the presence of flavonoids, phenols, tannins, steroids, amino acids, saponins, proteins, cardiac glycosides, and alkaloids. Glycoside and flavonoid contents were quantitatively estimated. Antioxidant activity pf methanolic extract of fruits was evaluated by studying ferric reducing antioxidant power assay using the standard procedure. The phytochemical analysis of fruit extracts of apple, avocado, pear, peach has revealed the presence of significant secondary metabolites such as steroids, cardiac glycosides, saponins, tannins, phenols, flavonoids, amino acid, and alkaloids. Quantitatively estimated glycoside content was higher in apple and least in avocado fruit extracts, and the flavonoid content was higher in avocado and least in peach fruit extract. The methanolic extract of apple, avocado, pear and peach fruits had shown significant ferric reducing antioxidant power assay. The fruit extracts were subjected to chromatographic fractionation followed by high-performance liquid chromatography. The major and sharp peaks for apple were found at 4 min while that of avocado lies between 1-2 min by using Gallic acid as standard.