Annals of Biological Sciences

Morphogenesis and histology of cultures of Iris ensata Thunb. generative organs

ANNUAL BIOTECHNOLOGY CONGRESS
August 17-18, 2017 | Toronto, Canada

L Tikhomirova

Altai State University, Russia

Posters & Accepted Abstracts: Ann Biol Sci

DOI: 10.21767/2348-1927-C1-003

Abstract

The initiation stage of Iris ensata Thunb. tissue cultures is optimized when flower fragments containing meristem tissue and with morphogenetic capacity are used. Direct regeneration of inflorescence axis (rachis) and perianth tube explants in tissue culture led to the formation of shoots typical of this species. Examination of the anatomical structures of the ovary, pistil and filament did not show any zones of meristematic activity, as examined over a 30-d cultivation period using these organs. These results could explain the lack of regenerative capacity of these explants.