Dentistry and Craniofacial Research

Effect of interleukin-33 on T-Helper17 cytokines InterleukinL-17 and InterLeukin-22 in saliva of patients with oropharyngeal candidiasis

EuroSciCon Conference on Dental & Dental Hygiene
March 26-27, 2018 Edinburgh, Scotland

Wifaq M.Ali Al-Wattar, Warkaa M.Al-Wattar, Maher K.Jasim and Jamal N.Ahmed

Assistant Prof. College of medicine, University of Baghdad,college of medicine,Unit of clinical and communicable diseases (MBCHB. MSc , FICMS) Lecturer in Oral Pathology department, College of Dentistry, Mystansiriyah University-Department of Oral pathology.. College of Dentistry, Baghdad University Professor ,Department of Oral Diagnosis, College of Dentistry, Baghdad University.

Posters & Accepted Abstracts: J Den Craniofac Res

DOI: 10.21767/2576-392X-C1-003

Abstract

Background: Oral candidiasis is a mycosis (yeast/fungal infection) of Candida species on the mucous membranes of the mouth. Humeral immune response to candidal infection has much theory according to site of infection mediated by T-helper maturation, differentiation and secretion of cytokines. Interleukin-33 is one of the IL-1 super families which are called alarming cytokines family as they inform the immune system about any tissue injury, infection or necrosis. IL-33 is multifunctional cytokines that has anti-inflammatory (protective), inflammatory effect on tissue by modulating the action of T-Helper17. Aim of study: To study the effect of InterLeukin-33 on T-Helper 17 cytokines InterLeukin-17 and InterLeukin-22 in saliva of patients with oropharyngeal candidiasis before and after therapy with Nystatine in comparison to control. Materials & Methods: forty patients visiting AL-Yarmouk teaching hospital /Baghdad were included in this study having clinical symptoms of oral candidiasis and after a positive confirmation of infection using slandered mycological techniques and identification to species using smearing methods and swab culturing on chromagar media, saliva samples were collected from them between 8:00 and 11:00 am and centrifuged and stored at -60 c0 until immunological analysis using ELISA kits. Results: there are a significant difference in median Of IL-17 in saliva of patients and control were respectively (85.4, 440.4), (p<0.001). Median value of IL-22 was higher in patients than control group was respectively (429.9, 38.3) and the difference was statistically significant with p 0.003. IL-33 median value in control group was lower than patient group were (227, 2 ��? 585, 2) respectively and statistically significant p value (<0.001). There are a great reduction in IL-17 and IL-22 after treatment with a median value of (-278.9, -134.35) respectively. At the same time IL-33 concentration elevated after therapy and the difference was (203.35) Conclusion: IL-33 has local protective effect (anti-inflammatory) on mucosa by decreasing the secretion of IL-17 and IL-22 in saliva of patients with oropharyngeal candidiasis and so reduces tissue damage caused by excessive immune response.

Biography

Warkaa M.Al-Wattar is a Lecturer in Oral Pathology department, College of Dentistry, Mystansiriyah University-Department of Oral pathology.
wifaqalwatar@rocketmail.com