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t h
E u r o S c i C o n C o n f e r e n c e o n
Neurology & Neurological
Disorders
Neurology 2018
J u l y 1 2 - 1 3 , 2 0 1 8
P a r i s , F r a n c e
Page 82
Journal of Neurology and Neuroscience
ISSN: 2171-6625
P
lant polysaccharides present some activities involving the central nervous
system, such as neuroprotective, antidepressant, antioxidant and anti-
inflammatory. We aim to evaluate the anticonvulsant and anti-inflammatory
effects of the polysaccharide rich extract fromG.
americana
leaves in mice. The
leaf dry powder (5 g) was depigmented in methanol and the polysaccharide-
rich extract (PRE) obtained by extraction with NaOH followed of precipitation
in absolute ethanol. PRE was dissolved in 0.9% NaCl and administered (9 mg/
kg) in male Swiss mice (25-35 g) by intraperitoneal (i.p.) route, 30 min before
seizures induced by a single dose of pentylenetetrazole (PTZ: 70 mg/kg, i.p),
n=7/group. The synergism of PRE effect was evaluated by its association with
diazepam (DZP: 0.01 mg/kg). After euthanasia, the prefrontal cortex (CPF),
hippocampus (HC) and striatum (EC) were removed for the quantification of
myeloperoxidase levels (MPO) by o-dianisidine method. Experimental protocol
was approved by Animal Ethics Committee (UECE Nº 2451142/2014). The PRE
increased the seizure latency (9 mg/kg: 171,7 ± 29,62
versus
saline: 62.00
± 4,709 s) and death latency (9 mg/kg: 597.4 ± 101,5
versus
saline: 150.0
± 14.52). The association of PRE with diazepam potentiated the protective
effect of DZP, increasing seizure latency (DZP: 128,3 ± 24,62 versus PRE + DZP:
222.4 ± 47.57), without altering in death latency. MPO levels was reduced in
hippocampus (PRE: 34.24 ± 7.167, DZP: 42.27 ± 9.559 and DZP + PRE: 31.26
± 5.726
versus
saline + PTZ: 81.91 ± 11.70) and striatum (PRE: 17,89 ± 3,310,
DZP + PRE: 18.69 ± 3.776
versus
saline + PTZ: 37.27 ± 5.169). However there
was no difference between groups (DZP, PRE or DZP + PRE) in each brain area.
We conclude that PRE of
G. americana
leaves protects against seizures and
promote anti-inflammatory effects in brain.
Sponsor's names: (CNPq/ CAPES/FUNCAP)
Biography
Edna Maria Camelo Chaves finished her doctorate at the age
of 6 at the Federal University of Ceará, in Pharmacology. She is
an Adjunct Professor at the State University of Ceará, working
in the Graduate Program in Physiology and Nursing. She has
published more than 13 articles in renowned magazines and
has served as a reviewer of scientific journals.
ednacam3@gmail.comEdna M. C. Chaves
1
, Dayanne T. T. Nonato
1
, Maria G. Pereira
1,2
,
Raquel M. C. B. Craveiro
1
, Anny B. S. Peixoto
1
, Letícia F. Lima
1
,
Samira L. Almeida
2
, Camila Fernandes
1
, Ana M. S. Assreuy
1
,
Gislei F. Aragao
3
1
Universidade Estadual do Ceará, Instituto Superior de Ciências Biomédicas- ISCB, Av. Dr. Silas
Munguba, 1700, Itaperi, 60714-903, Fortaleza, Brazil.
2
Laboratório de Polissacarídeos Bioativos, Faculdade de Educação Ciências e Letras do Sertão
Central (FECLESC/UECE), Quixadá-CE, Brazil.
3
Núcleo de Pesquisa e Desenvolvimento de Medicamentos (NPDM), Universidade Federal do
Ceará, Fortaleza, Brazil
Edna M. C. Chaves et al., J Neurol Neurosci 2018, Volume: 9
DOI: 10.21767/2171-6625-C1-009
Anticonvulsant and anti-inflammatory effects of the
polysaccharide rich extract from Genipa americana leaves