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t h
E u r o S c i C o n C o n f e r e n c e o n
Neurology & Neurological
Disorders
Neurology 2018
J u l y 1 2 - 1 3 , 2 0 1 8
P a r i s , F r a n c e
Page 81
Journal of Neurology and Neuroscience
ISSN: 2171-6625
V
oltage-dependent potassium channels (Kv1.3), play key role in a wide
variety of physiological processes, including immunity, metabolism and
the stabilization of the resting potential. In brain, activation of insulin receptor
is able to induce current suppression coupled to tyrosine phosphorylation of
Kv1.3 channel. Moreover, insulin can reduce the production of free radicals
and attenuate the inflammatory response. The Kv1.3 channel blockers, such as
neurotoxins isolated fromscorpion venom, are able to alter neuronal excitability
leading to neurological disorders accompanied by inflammatory response. The
aim of this study is to evaluate the neuroprotective effect of insulin injected
by intra-cerebro-ventricular (i.c.v.) route on neuro-inflammatory response and
oxidative stress induced by a blocker of Kv1.3 channel. The ability of insulin
to reduce the brain injuries, inflammatory response and oxidative stress
biomarkers induced by Kv1.3 channel blocker were assessed in NMRI mice at
24 h after co-injection of insulin and neurotoxin active on potassium channel.
Obtained results revealed that the central administration of insulin prevents
cerebral cortex injury, brain edema, cells infiltration and a change in the
permeability of the blood–brain barrier induced by the Kv1.3 channel blocker.
Insulin seems to also reduce significantly the pro-inflammatory cytokines (IL-
6, IL-17, TNF-α), MMP-2 and MMP-9 activities and oxidative stress markers
(H2O2, NO, MDA) in brain homogenates compared to those observed when
animals were injected with Kv1.3 channel blocker alone. These results indicate
that insulin is able to modulate the activity of potassium channels in brain by
modifying their properties, which probably prevent the binding of neurotoxin to
its receptor Kv channel and thus reduce the neuro-pathophysiological effects.
Biography
Zahida Taibi-Djennah has completed her PhD in Biochemistry-
Immunology and Innovative Biotherapies from University
of Sciences and Technology Houari Boumdiene, Faculty of
Biological Sciences, Laboratory of Cellular and Molecular
Biology. She is an Associate Professor level B at University
of Sciences and Technology Houari and is team member of
Biochemistry of Biomolecules: Mode of Action, Immunotherapy
and
Immunodiagnosi
(http://www.lbcm.usthb.dz/spip.php?rubrique4). She has published 5 papers in reputed
journals including Systemic Responses following Brain
Injuries and Inflammatory Process Activation Induced by a
Neurotoxin of
Androctonus
Scorpion Venom in the
Journal of
Neuroimmunomodulation
and Effect of cytokine antibodies
in the immunomodulation of inflammatory response and
metabolic disorders induced by scorpion venom in the
Journal
of International Immunopharmacology.
zahida.djennah@hotmail.comZ Taibi-Djennah and F Laraba-Djebari
USTHB-University of Science and Technology Houari Boumediene, Algeria
Z Taibi-Djennah et al., J Neurol Neurosci 2018, Volume: 9
DOI: 10.21767/2171-6625-C1-009
Effect of insulin on neuroinflammatory response and oxidative
stress induced by a blocker of Kv1.3 channel