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4 t h E u r o S c i C o n C o n f e r e n c e o n
Neurology & Neurological
Disorders
Neurology 2018
J u l y 1 2 - 1 3 , 2 0 1 8
P a r i s , F r a n c e
Page 85
Journal of Neurology and Neuroscience
ISSN: 2171-6625
Voltage-dependent potassium channels (Kv1.3), play key role in
a wide variety of physiological processes, including immunity,
metabolism and the stabilization of the resting potential. In brain,
activation of insulin receptor is able to induce current suppression
coupled to tyrosine phosphorylation of Kv1.3 channel. Moreover,
insulin can reduce the production of free radicals and attenuate
the inflammatory response. The Kv1.3 channel blockers, such as
neurotoxins isolated from scorpion venom, are able to alter neuronal
excitability leading to neurological disorders accompanied by
inflammatory response. The aim of this study is to evaluate the
neuroprotective effect of insulin injected by intra-cerebro-ventricular
(i.c.v.) route on neuro-inflammatory response and oxidative stress
induced by a blocker of Kv1.3 channel. The ability of insulin to reduce the
brain injuries, inflammatory response and oxidative stress biomarkers
induced by Kv1.3 channel blocker were assessed in NMRI mice at
24 h after co-injection of insulin and neurotoxin active on potassium
channel. Obtained results revealed that the central administration of
insulin prevents cerebral cortex injury, brain edema, cells infiltration
and a change in the permeability of the blood–brain barrier induced by
the Kv1.3 channel blocker. Insulin seems to also reduce significantly
the pro-inflammatory cytokines (IL-6, IL-17, TNF-α), MMP-2 and
MMP-9 activities and oxidative stress markers (H2O2, NO, MDA) in
brain homogenates compared to those observed when animals were
injected with Kv1.3 channel blocker alone. These results indicate that
insulin is able to modulate the activity of potassium channels in brain
by modifying their properties, which probably prevent the binding
of neurotoxin to its receptor Kv channel and thus reduce the neuro-
pathophysiological effects
Effect of insulin on neuroinflammatory response and oxidative
stress induced by a blocker of Kv1.3 channel
Z Taibi-Djennah and F Laraba-Djebari
USTHB-University of Science and Technology Houari Boumediene, Algeria
Z Taibi-Djennah et al., J Neurol Neurosci 2018, Volume: 9
DOI: 10.21767/2171-6625-C1-009
Biography
ZahidaTaibi-Djennah has completed her PhD in Biochemistry-Immunology
and Innovative Biotherapies from University of Sciences and Technology
Houari Boumdiene, Faculty of Biological Sciences, Laboratory of Cellular
and Molecular Biology. She is an Associate Professor level B at University
of Sciences and Technology Houari and is team member of Biochemistry
of Biomolecules: Mode of Action, Immunotherapy and Immunodiagnosi
). She has published 5
papers in reputed journals including Systemic Responses following Brain
Injuries and Inflammatory Process Activation Induced by a Neurotoxin of
Androctonus Scorpion Venom in the Journal of Neuroimmunomodulation
and Effect of cytokine antibodies in the immunomodulation of
inflammatory response and metabolic disorders induced by scorpion
venom in the Journal of International Immunopharmacology.