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Mycology 2017
September 25-26, 2017
conferenceseries
.com
September 25-26, 2017 Chicago, USA
2
nd
International Conference on
Mycology & Mushrooms
Volume 3, Issue 2 (Suppl)
Med Mycol Open Access
ISSN: 2471-8521
Distribution of amatoxins and phallotoxins in different tissues and development stages of
Amanita
subpallidorosea
Shuo Zhang
1
, Haijiao Li
2
, Dawei Chen
1
, Yunfeng Zhao
1
and
Chengye Sun
2
1
China National Center for Food Safety Risk Assessment, China
2
Chinese Center for Disease Control and Prevention, China
A
manita subpallidorosea
is a lethal mushroom discovered recently in China. Due to the morphological similarity to edible
mushrooms and the high toxicity,
A. subpallidorosea
had caused severe mushroom poisonings in China. However, the contents
and distribution of the major toxins in
A. subpallidorosea
remain poorly studied. In this study, the concentration of the major
cyclopeptide toxins, amatoxins and phallotoxins in different tissues and development phases were systematically analyzed for the first
time. To find other structure related high risk compounds which are not reported or available as standards to launch toxicological
study in
A. subpallidorosea
, a new non-targeted strategy based on liquid chromatography-high resolution mass spectrometer (LC-
HRMS) was applied to analyze the toxin profiling of the mushroom and to find new cyclopeptide toxins. The results showed that the
concentration of the total amatoxins in
A. subpallidorosea
were remarkably high, which was much higher than the worldly notorious
A. phalloides
, a lethal species from Europe and North America. The distribution of amatoxins and phallotoxins in different tissues
showed the highest concentrations of amatoxins and phallotoxins were found in the cap and the lowest concentrations in the volva.
Further analysis of mushrooms in different development stages showed that the amatoxin content was relatively high during early
development, in which stage the fruit body grew most vigorously and regarded as tasty stage for mushroom picking. With the LC-
HRMS based strategy, seven cyclopeptides and two new compounds were found and confirmed by parallel reaction monitoring
(PRM) and all ion fragmentation (AIF) mode on a high resolution hybrid quadrupole-orbitrap mass spectrometer.
zhangshuo6789@sina.comMed Mycol Open Access, 3:2, 2017
DOI: 10.21767/2471-8521-C1-003