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Mycology 2017
September 25-26, 2017
conferenceseries
.com
September 25-26, 2017 Chicago, USA
2
nd
International Conference on
Mycology & Mushrooms
Volume 3, Issue 2 (Suppl)
Med Mycol Open Access
ISSN: 2471-8521
The antifungal effects of alcoholic extract of
Ganoderma lucidum
on candida isolates
Javaher Chabavizadeh
1
, Mojgan Sadeghi
1
, Mostafa Chadeganipour
1
, Mohammad Ghahri
2
, Parvin Dehghan
1
and
Behzad Mahaki
1
1
Isfahan University of Medical Sciences, Iran
2
Imam Hossain School of Basic Sciences, Iran
Introduction:
Candidiasis is a common fungal infection caused by various species of the
Candida. Candida albicans
is the most
important etiologic agent of candidiasis which includes about 60%-75% of this disease. The extent of fungal opportunistic infections
in susceptible individuals on one hand and increasing drug resistance on the other hand, leads to the importance of antifungal effects
of traditional plant products. One of the edible mushrooms which is named as the best medicinal fungi, having many health benefits
and various therapeutic properties is named
Ganoderma lucidum
. In this study, the therapeutic importance of this mushroom, as well
as previous studies for antifungal and antibacterial properties of this fungus, was designed.
Materials & Methods:
This study was carried out on patients with candidemia admitted to some specialized hospitals in Tehran. To
identify the
C.albicans
specie, out of 4850 blood cultures, 43 cases were identified as candidiasis, by using phenotypic and molecular
methods,
in vitro
. Then microdilution methods were used to prepare different concentrations of
G. lucidum
ethanol extract to
determined MIC (minimum inhibitory concentration) and MFC (minimum fungicidal concentration) for each
C.albicans
isolated
species as well.
Findings & Conclusion:
The result showed that out of 43 candidiasis, the frequency of candida isolates were as follows:
C. albicans
22
(52%),
C. parapsilosis
10 (23%),
C. glabrata
8 (18%) and
C. tropicalis
3 (7%) respectively. By microdilution method the concentration
of 5.2 mg/ml inhibited most species. The MIC was 3.1 mg/ml and the maximum concentration was 10.4 mg/ml. The MFC was 5.2
mg/ml and the maximum concentration was 20.8 mg/ml. According to the results of this study, the
G. lucidum
ethanol extract can be
used as an antifungal product in the future studies to lead for better control and treatment of candidiasis.
chabavizadeh@med.mui.ac.irMed Mycol Open Access, 3:2, 2017
DOI: 10.21767/2471-8521-C1-003