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Mycology 2017
September 25-26, 2017
conferenceseries
.com
September 25-26, 2017 Chicago, USA
2
nd
International Conference on
Mycology & Mushrooms
Volume 3, Issue 2 (Suppl)
Med Mycol Open Access
ISSN: 2471-8521
Mycoflora and aflatoxin levels of edible vegetable oils sold in Nigeria and possible control measures using
imarsil and activated charcoal
Flora Oluwafem
1
, Oni E O
1
and
Kolapo A L
2
1
Federal University of Agriculture, Nigeria
2
The Polytechnic, Ibadan, Nigeria
E
dible oils play vital role in a well-balanced diet. However, the infestation of most edible oils by mycotoxigenic moulds possesses
high health risk for humans and animals. It thus necessitates the need to screen the oils and detoxify those using local and
inexpensive adsorbents. Ten samples of edible vegetable oils from different plant sources such as canola, palm-kernel, sunflower,
olive, groundnut, soya-beans, coconut, cotton seed, palm and corn oils were purchased from Nigerian markets and assessed for
fungi and aflatoxins levels using standard microbiological procedures and High-Performance Liquid Chromatography (HPLC)
respectively. Adsorption studies of Aflatoxins (AF) were performed on the AF positive oils using
imarsil
and activated charcoal at 2
and 3 % concentrations. Sensory evaluation of treated and untreated oils was also carried out using 10 members panel. Prevalence
of isolated fungi were:
Aspergillus fumigatus
(43%),
Mucor sp
(17.9%), Saccharomyces sp (10%), A. niger (7.1%), A. oryzae (7.1%),
A. flavus
(7.1%), Penicillium sp. (7.1%) and Rhizopus sp. (3.6%). Seven samples were positive for AF. Cotton oil, Sun-flower oil and
Canola oil had no detectable AF levels while Corn oil, Coconut oil, Olive oil, Soya oil, Palm kernel oil, Palm oil and Groundnut oil
had the following aflatoxin concentrations respectively ; 157ng/kg, 49ng/kg, 33ng/kg, 28ng/kg, 9ng/kg, 5ng/kg and 4ng/kg. At ≤ 9
ng/L AF contamination rate, both
imarsil
and activated charchoal exhibited 100 % adsorption efficiency within one hour. At AF
contamination rates of 28-157 ng/L, activated charcoal was not effective while
imarsil
had 100 % removal efficiency within 3 hours.
Sensory evaluation results showed
imarsil
-treated vegetable oil had good organoleptic properties while activated charcoal –treated
vegetable oils had off-flavour. Aflatoxins present in some vegetable oils can be eliminated using
imarsil
.
foluwafemi2000@yahoo.co.ukMed Mycol Open Access, 3:2, 2017
DOI: 10.21767/2471-8521-C1-003