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Mycology 2017
September 25-26, 2017
conferenceseries
.com
September 25-26, 2017 Chicago, USA
2
nd
International Conference on
Mycology & Mushrooms
Volume 3, Issue 2 (Suppl)
Med Mycol Open Access
ISSN: 2471-8521
Expression of the
Aspergillus fumigatus
α-1, 2-mannosidase gene (
MsdS
) in
Trichoderma reesei
affects cell
wall synthesis and polarized growth
Prakriti Sharma Ghimire
Chinese Academy of Sciences, China
Statement of the problem:
α-1, 2-mannosidase is a very important enzyme, essential for N-glycan processing and plays a significant
role in the biosynthesis and organization of fungal cell wall. Lack of α-1, 2-mannosidase has been observed to cause cell wall defect in
yeast and filamentous fungi.
Trichoderma reesei
is known to be non-toxic to human, and its N-glycan onmature secretory glycoprotein
is GlcNAc2Man8, which is different from the GlcNAc2Man6 in the wild-type
Aspergillus fumigatus
and similar to the glycoform of
the
MsdS
deletion mutant, which was characterized by cell wall defect and polarized growth.
Methodology & Theoretical Orientation:
To gain insight into the physiological function of the N-glycan processing in
T. reesei
, in
this study the
A. fumigatus
α-1, 2-mannosidase MsdS was introduced into
T. reesei
.
Findings:
The mutant strain expressing MsdS produced a major glycoform of GlcNAc2Man6 on its secretory glycoproteins, instead
of GlcNAc2Man8 in the wild-type. Although the cell wall content of the mutant was changed, it appeared that its cell wall integrity
was not affected. However, multiple budding and random branching was observed in the mutant. In addition, the mutant showed
less dense filamentous material on cell surface under favorable growth condition, while a complete loss of filamentous material
accompanied by an increase of cell wall chitin was observed at elevated temperatures.
Significance & Conclusion:
Our results, for the first time, indicated that processing of the N-glycan might play a major role in
sorting and polarized transportation of certain glycoproteins in
T. reesei
, which is vital for the cell wall synthesis and polarity. Further,
our results also indicated that the mechanism of the N-glycan-related sorting of glycoprotein in
T. reesei
is different from that in
A.
fumigatus
.
sharprakriti@gmail.comMed Mycol Open Access, 3:2, 2017
DOI: 10.21767/2471-8521-C1-003