18 / 25
Page 61
Notes:
Mycology 2017
September 25-26, 2017
conferenceseries
.com
September 25-26, 2017 Chicago, USA
2
nd
International Conference on
Mycology & Mushrooms
Volume 3, Issue 2 (Suppl)
Med Mycol Open Access
ISSN: 2471-8521
Isolation and molecular identification of
Trichophyton mentagrophytes
from patients with dermatophytosis
Hana K Maikhan
1
, Shwan K Rachid
2
and
Khalid A Habeb
3
1
University of Garmian, Iraq
2
Charmo University, Iraq
3
University of Baghdad, Iraq
O
ut of 30 clinical specimens isolated from patients with dermatophytosis in the Dermatology unit of the General Hospital in
Kalar distric\Sulaimania province\ North region of Iraq, five clinical isolates showed positive results for dermatophytes, four
of them were identified as
Trichophyton mentagrophytes
characterized by the production of white colonies at the surface and brown
color at the reverse. Primarily, the colonies appeared with cottony texture and after two weeks changed to powdery-granular colonies.
Microscopic examination appeared numerous single-celled, spherical shaped microconidia were seen as clustered on both sides of
hyphae. Multiseptate cigar shaped macroconidia and spiral hyphae were seen during the formation of granular colonies.
Trichophyton
mentagrophytes
was positive for urease and hair perforation testes. Molecular identification according to the conventional PCR by
using set primers ITS1 and ITS4 resulted in PCR product about 700bp in all isolates. PCR-RFLP by using
BstN1
digestion enzyme
revealed four pattern bands 250,180,150 and 120bp. Sequencing of the ITS region in one of the isolates,
Trichophyton mentagrophytes
revealed similarity of about 86% with
Trichophyton mentagrophytes
isolate ATCC11481 regarding the following parameters: internal
transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene, complete sequence; and internal transcribed spacer 2, partial
sequence. 2) Sequencing Results: Sequencing of the ITS region in one of the isolated
T.mentagrophytes
revealed similarity about 86%
with
Trichophyton mentagrophytes
isolate ATCC11481 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene,
complete sequence; and internal transcribed spacer 2, partial sequence.
hana.kareem@garmian.edu.krdMed Mycol Open Access, 3:2, 2017
DOI: 10.21767/2471-8521-C1-003